Comparison of the Potency of Pterostilbene with NF-?B Inhibitors in Platelet Activation: Mutual Activation by Akt-NF-?B Signaling in Human Platelets

Myocardial infarction and cerebral ischemic stroke are prominent causes of death worldwide. Platelets play major roles in these diseases, although they anucleated cells, but also express the NF-?B. Pterostilbene (PTE) possesses some intriguing pharmacological properties, including capacity to inhibit platelet activation. We investigated inhibitory role PTE NF-?B-mediated signal events compared relative potency with that classical NF-?B inhibitors. I?B kinase (IKK) inhibitor, BAY11-7082, proteasome Ro106-9920, inhibited aggregation; activity BAY11-7082 were similar, Ro106-9920 was weak this reaction. diminished signaling molecules, IKK, I?B?, p65 phosphorylation, reversed I?B? degradation. However, only effective diminishing phosphorylation reversing In investigating Akt cell events, MK-2206 (an inhibitor Akt) markedly abolished IKK phosphorylation; reduced phosphorylation. exhibited more potent vivo than did acute pulmonary thromboembolism. conclusion, we identified a distinctive activation pathway involved PTE-mediated antiplatelet aggregation, demonstrated powerful as prophylactic clinical therapy for cardiovascular diseases.